Journal: Microorganisms

Article Title: Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

doi: 10.3390/microorganisms10030526

Figure Lengend Snippet: Three-dimensional reconstruction of sessile biofilm formation on titanium surface by means of CLSM. ( A ) Inclined view and ( B ) top view of biofilm grown in the presence of laboratory medium BHI or in physiological fluids, plasma ( left ) and bovine synovial fluid (BSF, right ). Upper panels show images acquired with a 20× objective; lower panels show images acquired with a 5× objective.

Article Snippet: Planktonic and sessile growth was performed throughout the work in BHI broth (Merck), human plasma, and bovine synovial fluid (BSF, Lampire Biological Laboratories).

Techniques:

Journal: Microorganisms

Article Title: Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

doi: 10.3390/microorganisms10030526

Figure Lengend Snippet: CLSM quantitative analysis of biofilm volume and surface on titanium disks demonstrates a reorganization of the sessile architecture and biomass distribution. ( A ) Total surface occupied by sessile biofilm on titanium disk. Horizontal lines represent statistical significance (Wilcoxon, p < 0.05 ). ( B ) Total volume of biofilm biomass. ( C ) Average volume/surface ratio in the different media. Black bars represent biofilm grown in laboratory medium BHI, gray and white bars represent biofilm grown in physiological fluids, respectively, plasma and BSF.

Article Snippet: Planktonic and sessile growth was performed throughout the work in BHI broth (Merck), human plasma, and bovine synovial fluid (BSF, Lampire Biological Laboratories).

Techniques:

Journal: Microorganisms

Article Title: Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

doi: 10.3390/microorganisms10030526

Figure Lengend Snippet: Pattern formation analysis reveals significant differences between lab and physiological media. ( A ) Representative CLSM images of sessile biofilm attachment to a titanium disk submerged in laboratory medium (BHI) or in physiological media (plasma, BSF). The 15 × 15 grid used for analysis is also shown. ( B ) Heatmap showing the biofilm density in each grid element. ( C ) Probability density function of grid element biofilm density for the same medium and different oxygen availability and motion stress conditions. Grid element biofilm density was normalized with respect to the mean biofilm density of each image to highlight changes in biofilm distribution patterns independently of mean biofilm growth. The insets show PDFs averaged across oxygen availability and motion stress conditions and report the median of the distribution. ( D ) Probability density function of grid elements biofilm density for different media and same oxygen availability and motion stress conditions.

Article Snippet: Planktonic and sessile growth was performed throughout the work in BHI broth (Merck), human plasma, and bovine synovial fluid (BSF, Lampire Biological Laboratories).

Techniques:

Journal: Microorganisms

Article Title: Host Environment Shapes S. aureus Social Behavior as Revealed by Microscopy Pattern Formation and Dynamic Aggregation Analysis

doi: 10.3390/microorganisms10030526

Figure Lengend Snippet: Fourier analysis reaffirms significant differences in biofilm pattern formation between lab and physiological media. ( A ) Representative CLSM images of sessile biofilm attachment to a titanium disk submerged in laboratory medium (BHI) or in physiological media (plasma, BSF). ( B ) Two-dimensional Fourier power spectrum of the representative CLSM images in ( A ). ( C ) CSPfs for the same medium in different oxygen availability/motion stress conditions. ( D ) CSPfs for the same oxygen availability/motion stress condition in different media.

Article Snippet: Planktonic and sessile growth was performed throughout the work in BHI broth (Merck), human plasma, and bovine synovial fluid (BSF, Lampire Biological Laboratories).

Techniques: